Green Synthesis of Mn-Doped ZnO Nanoparticles: Characterization, Hemocompatibility, and Cytotoxicity Effect Against C540 Melanoma Cells
Keywords:
Mn-Zn-NP, Prosopis fracta, Hemolysis, Melanoma cancer cells, MTTAbstract
In this study, we present a rapid and eco-friendly method for the preparation of ZnO nanoparticles (Zn-NP) and 5% Mn-doped ZnO nanoparticles (Mn-Zn-NP) using aqueous extract of Prosopis fracta. The synthesized nanoparticles were characterized using PXRD, FESEM, EDX, UV-Vis, DLS, FT-IR, and Raman spectroscopy. PXRD analysis confirmed the hexagonal wurtzite structure of both samples, with crystallite sizes of 35.16 nm and 39.52 nm for Zn-NP and Mn-Zn-NP, respectively. The successful doping of Mn2+ ions into the ZnO lattice was demonstrated by shifts in the PXRD peaks, EDX elemental analysis (4.52% Mn) and a blue shift in the UV-Vis absorption from 368 nm to 339 nm. FESEM imaging revealed a spherical morphology with particle sizes of ~30 nm (Zn-NP) and ~50 nm (Mn-Zn-NP). Excellent hemocompatibility was demonstrated for both samples using hemolysis assay. MTT cytotoxicity assay against C540 melanoma cells revealed concentration-dependent cell death, with Mn-Zn-NP presenting significantly higher toxicity (IC50 ~25 μg/mL) compared to undoped Zn-NP (IC50 ~50 μg/mL). Our findings indicate that manganese doping significantly improves the anticancer potential of ZnO-NP and suggest Mn-Zn-NP as a promising candidate for melanoma treatment.
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